Giardia lamblia growth inhibition by action of the aqueous and methanolic extract of cucurbita pepo seed
Abstract
Giardia lamblia is the protozoan parasite that causes giardiasis, which is characterized by abdominal discomfort, weight loss and malnutrition. The drug of choice for treatment is metronidazole however, presents several adverse side effects in the patient. Moreover, in the herbalist is known seed Cucurbita pepo by desparasitante properties, mainly Entamoeba histolytica and Taenia solium. Therefore it was hypothesized that the seeds of C. pepo contain active substances which inhibit the growth of G. lamblia axenic in vitro conditions. The objective of this study was to evaluate the biological activity of aqueous and methanolic extract of the seeds of C. pepo on the growth of G. lamblia. Both aqueous methanol extract as identified seven functional groups and antioxidant activity was not significant. The methanol extract showed higher aqueous followed giardicida effect, this difference may be due to the saponins found only in the methanol extract. The results suggest that the seeds contain active ingredients that can be used for research into new treatments for giardiasis.
Downloads
References
Adam R.D., (2001). Biology of Giardia lamblia. Clinical Microbiology Reviews. 14(3), 447-475.
Becerril M.A., (2008). Parasitología Médica. México: Mc Graw Hill.
Botero D. & Restrepo M., (1998). Giardiasis. Parasitosis humana. Colombia: Corporación para Investigaciones Biológicas.
Cedillo R. y Muñoz O., (1992). In vitro susceptibility of Giardia lamblia to albendazole, mebendazole and other chemotherapeutic agents. Journal o Medicine Microbiology. 37, 221-224.
Cifuentes E., Suarez L., Espinosa M., Juarez-Figueroa L., Martinez P., (2004) A. Risk of Giardia intestinalis infection in children from an artificially recharged groundwater area in Mexico city. American Journal of Tropical Medicine and Hygiene. 71, 65-70.
Cordingley F.T., Crawford G.P., (1986). Giardia infection causes vitamin B12 deficiency. Australian and New Zealand. Journal of Medicine. 16, 78-79.
Diamond L.S., Harlow D. & Cunnick C.C., (1978). A new medium for the axenic cultivation of Entamoeba histolytica and other Entamoeba. Transactions of the Royal Society of Tropical Medicine. 72, 431-431.
Domínguez, X.A., (1973). Métodos de investigación fitoquímica. México, D.F.: Editorial LIMUSA.
Gillon J., (1985). Clinical studies in adult presenting with giardiasis to a gastro-intestinal unit. Scottish Medical Journal. 30, 89-95.
Gopal H.B.; Davila G.W, de la Rosette J.M.C.H., (2009). Continence Current concepts and treatment strategies. USA: SPRINGER.
Guerrant R.L., Walker D.H., Weller P.F., (2002). Enfermedades Infecciosas Tropicales. España: ELSEVIER Science.
Huang D.B. & White A.C. (2006). An updated review on Cryptosporidium and Giardia. Gastroenterology Clinics of North America. 35(2), 291-314.
Legator M.S. Connor, T.H. & Stoeckel, M., (1975). Detection of mutagenic activity of metronidazole and niridazole in body fluids of human and mice. Science. 188, 1118-1119.
Rodríguez F.G., Cancino B.L.,Prieto G.E., Espinosa A.J., (2001). Tinidazol: Una droga antimicrobiana con actividad genotóxica. Revista Cubana de Investigaciones Biomédicas. 20(1), 54-58.
Tay J., Velasco O., Lara R., Gutiérrez M., (1996). Giardiasis. Parasitología Médica. México: Méndez Cervantes.
In order to promote the development and dissemination of research in education in Latin America, the Ibero-American Journal for Educational Research and Development (RIDE) adhered to the Budapest Open Access Initiative, which is why it is identified as a Open access publication. This means that any user can read the complete text of the articles, print them, download them, copy them, link them, distribute them and use the contents for other purposes. Creative Cummons licenses allow users to specify the rights to use an open access journal available on the Internet in such a way that users know the rules of publication. Authors who publish in this journal accept the following conditions: Authors they keep the author's rights and give the magazine the right of the first publication, with the work registered with the attribution license of Creative Commons, which allows third parties to use the published material whenever they mention the authorship of the work and the first publication in this The authors can make other independent and additional contractual agreements for the non-exclusive distribution of the version of the article published in this journal (eg, include it in an institutional repository or publish it in a book) as long as they clearly indicate that The work was published for the first time in this magazine. Authors are allowed and recommended to publish their work. low on the Internet (for example on institutional or personal pages) before and during the review and publication process, as it can lead to productive exchanges and to a greater and faster dissemination of the published work
